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Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
《工程(英文)》 doi: 10.1016/j.eng.2023.02.016
关键词: Glycoproteins N-glycans Reversible labeling Hydrophilic interaction liquid chromatography Capillary gel electrophoresis Mass spectrometry
可去除染料——N-聚糖多方法深入分析中的缺失环节 Article
Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp
《工程(英文)》 2023年 第26卷 第7期 页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.
Jian LU,Paul S. WILLS,P. CHRIS WILSON
《环境科学与工程前沿(英文)》 2016年 第10卷 第3期 页码 477-481 doi: 10.1007/s11783-015-0820-z
关键词: off-flavor/odor compounds liquid-liquid microextraction gas chromatography-tandem mass spectrometry
Liang ZHU, Jingkang WANG
《化学科学与工程前沿(英文)》 2013年 第7卷 第3期 页码 322-328 doi: 10.1007/s11705-013-1348-z
关键词: tobramycin aminoglycoside refractive index detector high-performance liquid chromatography ion-pairing
WEI Yimei,ZHANG Yuan,XU Jian,GUO Changsheng,LI Lei,FAN Wenhong
《环境科学与工程前沿(英文)》 2014年 第8卷 第3期 页码 357-371 doi: 10.1007/s11783-013-0580-6
关键词: antibiotics liquid chromatography–tandem mass spectrometry (LC-MS/MS) water sediment fish muscle
Feng Zhu, Zhijian Yao, Wenliang Ji, Deye Liu, Hao Zhang, Aimin Li, Zongli Huo, Qing Zhou
《环境科学与工程前沿(英文)》 2020年 第14卷 第3期 doi: 10.1007/s11783-020-1228-y
关键词: Hydrophilic resin Solid phase extraction Pharmaceuticals and personal care product Ultra-performance liquid chromatography and tandem mass spectrometry Environmental water
Shengguo DENG, Zeyuan DENG, Yawei FAN, Jing LI, Rong LIU, Dongmei XIONG,
《化学科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 375-382 doi: 10.1007/s11705-009-0247-9
关键词: two-phase-solvent flavonoid glycoside high-speed counter-current macroporous counter-current chromatography
Chengkun WANG, Xiaojian ZHANG, Jun WANG, Chao CHEN
《环境科学与工程前沿(英文)》 2012年 第6卷 第6期 页码 770-777 doi: 10.1007/s11783-012-0412-0
关键词:
《环境科学与工程前沿(英文)》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1338-6
• N-Cl-DCAM, an emerging N-DBP in drinking water was investigated.
关键词: N-chloro-2 2-dichloroacetamide Liquid chromatography with tandem mass spectrometry Precursors Removal efficiency Ozonation integrated with biological activated carbon
Yue-E SUN, Wenshui XIA, Xueyan TANG, Zhiyong HE, Jie CHEN,
《化学科学与工程前沿(英文)》 2009年 第3卷 第4期 页码 407-412 doi: 10.1007/s11705-009-0255-9
关键词: high-performance liquid tension different spectrometry chromatography
Hydrophilic/underwater superoleophobic graphene oxide membrane intercalated by TiO
Zhichao Wu, Chang Zhang, Kaiming Peng, Qiaoying Wang, Zhiwei Wang
《环境科学与工程前沿(英文)》 2018年 第12卷 第3期 doi: 10.1007/s11783-018-1042-y
关键词: Hydrophilic Superoleophobic Graphene oxide Membrane Titanium dioxide nanotubes Oil-water separation
Yong QIU, He JING, Hanchang SHI,
《环境科学与工程前沿(英文)》 2010年 第4卷 第3期 页码 301-310 doi: 10.1007/s11783-010-0236-8
关键词: surface and tap water perfluorocarboxylic acids (PFCAs) perfluoroalkyl sulfonates (PFASs) Lake Taihu area ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS)
Heng Fang, Aihua Zhang, Xiaohang Zhou, Jingbo Yu, Qi Song, Xijun Wang
《医学前沿(英文)》 2020年 第14卷 第5期 页码 651-663 doi: 10.1007/s11684-019-0709-5
关键词: metabolomics liquid chromatography-mass spectrometry metabolites metabolic pathways
Eniko Haaz, Botond Szilagyi, Daniel Fozer, Andras Jozsef Toth
《化学科学与工程前沿(英文)》 2020年 第14卷 第5期 页码 913-927 doi: 10.1007/s11705-019-1877-1
关键词: hydrophilic pervaporation non-ideal mixture modelling extractive heterogeneous-azeotropic distillation heat integration
Experimental investigations of frost release by hydrophilic surfaces
Zhongliang LIU, Lingyan HUANG, Yujun GOU, Yaomin LIU
《能源前沿(英文)》 2010年 第4卷 第4期 页码 475-487 doi: 10.1007/s11708-010-0114-x
关键词: frost formation hydrophilicity heat and mass transfer performance
标题 作者 时间 类型 操作
Removable Dyes—The Missing Link for In-Depth N-glycan Analysis via Multi-Method Approaches
Samanta Cajic,René Hennig,Valerian Grote,Udo Reichl,Erdmann Rapp,
期刊论文
Trace analysis of off-flavor/odor compounds in water using liquid-liquid microextraction coupled withgas chromatography—positive chemical ionization-tandem mass spectrometry
Jian LU,Paul S. WILLS,P. CHRIS WILSON
期刊论文
Fast determination of tobramycin by reversed-phase ion-pair high performance liquid chromatography with
Liang ZHU, Jingkang WANG
期刊论文
Simultaneous quantification of several classes of antibiotics in water, sediments, and fish muscles by liquidchromatography–tandem mass spectrometry
WEI Yimei,ZHANG Yuan,XU Jian,GUO Changsheng,LI Lei,FAN Wenhong
期刊论文
An efficient resin for solid-phase extraction and determination by UPLCMS/MS of 44 pharmaceutical personal care products in environmental waters
Feng Zhu, Zhijian Yao, Wenliang Ji, Deye Liu, Hao Zhang, Aimin Li, Zongli Huo, Qing Zhou
期刊论文
Application of high-speed counter-current chromatography coupled with high performance liquid chromatography
Shengguo DENG, Zeyuan DENG, Yawei FAN, Jing LI, Rong LIU, Dongmei XIONG,
期刊论文
Characteristics and removal mechanism of the precursors of N-chloro-2,2-dichloroacetamide in a drinking water treatment process at Taihu Lake
期刊论文
Effects of fatty acid chain length and degree of unsaturation on the surface activities of monoacyl trehaloses
Yue-E SUN, Wenshui XIA, Xueyan TANG, Zhiyong HE, Jie CHEN,
期刊论文
Hydrophilic/underwater superoleophobic graphene oxide membrane intercalated by TiO
Zhichao Wu, Chang Zhang, Kaiming Peng, Qiaoying Wang, Zhiwei Wang
期刊论文
Perfluorocarboxylic acids (PFCAs) and perfluoroalkyl sulfonates (PFASs) in surface and tap water around Lake Taihu in China
Yong QIU, He JING, Hanchang SHI,
期刊论文
High-throughput metabolomics reveals the perturbed metabolic pathways and biomarkers of Yang Huang syndrome as potential targets for evaluating the therapeutic effects and mechanism of geniposide
Heng Fang, Aihua Zhang, Xiaohang Zhou, Jingbo Yu, Qi Song, Xijun Wang
期刊论文
Combining extractive heterogeneous-azeotropic distillation and hydrophilic pervaporation for enhanced
Eniko Haaz, Botond Szilagyi, Daniel Fozer, Andras Jozsef Toth
期刊论文